Faculty Profile

Timothy A Mietzner, Ph.D.

College of Osteopathic Medicine
Education Oregon Health Sciences University
Campus Location
Seton Hill, PA
Ph:
View On Google Maps

Biography

Biographical Sketch CURRICULUM VITAE NAME:  Timothy A. Mietzner HOME ADDRESS:   1341 Cordova Road                                                                        Pittsburgh, PA  15206 HOME PHONE:        (412) 363-6409   CITIZENSHIP:  U.S. Citizen BUSINESS ADDRESS:          Lake Erie College of Osteopathic Medicine at Seton Hill 20 Seton Hill Drive Greensburg, PA 17601 BUSINESS PHONE:              (724)552-2891 (office) (412)215-6700 (cell phone) FAX NUMBER:                      (724)552-2865 EMAIL:                                  tmietzner@lecom.edu tamietzner@gmail.com
PPUBLICATIONS
I.  Refereed Articles 1.  McFarland, L., Mietzner, T.A., Knapp, J.S., Sandström, E.C., Holmes, K.K., and Morse, S.A. (1983) Gonococcal sensitivity to fecal lipids can be mediated by an Mtr-independent mechanism.  J. Clin. Microbiol. 18:121-127. 2.  Mietzner, T.A., Luginbuhl, G.L., Sandström, E.C., and Morse, S.A. (1984) Identification of an iron-regulated 37,000 dalton protein in the cell envelope of Neisseria gonorrhoeae.  Infect. Immun. 45:410-416. 3.  Mietzner, T.A. and Morse, S.A.  (1985) The iron-regulated membrane proteins of Neisseria gonorrhoea:  Purification and partial characterization of a 37,000 dalton protein.  In: The pathogenic Neisseria.  Schoolnik, G.K., Brooks, G.F., Falkow, S., Knapp, J.S., McCutchan, A. and Morse, S.A., eds.  American Society for Microbiology, Washington, D.C., pp. 406-415. 4.  Mietzner, T.A., Barnes, R.C., Jeanlouis, Y.A., Shafer, W.M., and Morse, S.A. (1986) Distribution of an antigenically related iron-regulated protein among the Neisseria spp.  Infect. Immun. 51:60-68. 5.  Gariépy, J., Mietzner, T.A., and Schoolnik, G.K.  (1986) Peptide antisera as sequence-specific probes of protein conformational transitions:  Calmodulin exhibits calcium-dependent changes in antigenicity.  Proc. Natl. Acad. Sci. U.S.A. 83: 8888-8892. 6.  Mietzner, T.A., Bolan, G., Schoolnik, G.K., and Morse, S.A. (1987) Purification and characterization of the major iron-regulated protein expressed by pathogenic Neisseriae.  J. Exp. Med. 165:1041-1057. 7.  Fohn, M.J., Mietzner, T.A., Hubbard, T.W., Morse, S.A., and Hook, E.W.  (1987) Human IgG antibody response to the major gonococcal iron-regulated protein.  Infect. Immun. 55:3065-3069. 8.  Morse, S.A., Mietzner, T. A., Bolan, G., Le Faou, A. and Schoolnik, G.K.  (1987) Characterization of the major iron-regulated protein of Neisseria gonorrhoeae and Neisseria meningitidis.  A. VanLeeuw 53:465-469. 9.  Morse, S.A., Chen, C.Y., Le Faou, A. and Mietzner, T.A.  (1988) A potential role for the major iron-regulated protein expressed by pathogenic Neisseria spp.  Reviews of Infect. Dis. 10 (supp):306-310. 10.  Berish, S.A., Mietzner, T.A., Genco, C.A., Hollaway, B.P., Maier, L.W., and Morse, S.A.  (1990) Molecular cloning and characterization of the structural gene for the major iron-regulated protein expressed by Neisseria gonorrhoeae.  J. Exp. Med. 171:1535-1546. 11.  Ho, A.S.Y., Mietzner, T.A., Smith, A.J., and Schoolnik, G.K. (1990) The pili of Aeromonas hydrophila:  Identification of an environmentally regulated "mini-pilin".  J. Exp. Med.172:795-806. 12.  Hanna, P.C., Mietzner, T.A., Schoolnik, G.K., and McClane, B.A. (1991) Localization of the receptor-binding region of Clostridium perfringens enterotoxin utilizing cloned toxin fragments and synthetic peptides:  The 30 C-terminal amino acids define a functional binding region.  J. Biol. Chem. 266:11037-11043. 13.  Morse, S.A., Berish, S.A., Chen, C.-Y., Trees, D.L., Mietzner, T.A., Genco, C.A. and Kapczynski, D.  (1991)  Structure, function, and regulation of the neisserial iron-binding protein, Fbp. Proceedings of the seventh conference on pathogenic Neisseria. In: Neisseriae 1990.  Achtman, M., Kohl, P., Marchal, C., Giovanna, M., Seiller, A., and Theisen, B., eds.  Walter de Gruyter, New York, pp. 453-458. 14.  Hanna, P.C., Wieckowski, E.U., Mietzner, T.A., and McClane, B.A. (1992) Molecular mapping of functional regions of Clostridium perfringens type A enterotoxin.  Infect. Immun. 60:2110-2114. 15.  Berish, S.A., Chen, C.-Y., Mietzner, T.A., and Morse, S.A.  (1992) Expression of a functional Neisserial Fbp gene in Escherichia coli.  Molec. Microbiol. 6:2607-2615. 16.  Mietzner, T.A., Kokai-Kun, J.F., Hanna, P.C., and McClane, B.A. (1992) A conjugated synthetic peptide corresponding to the C-terminal region of Clostridium perfringens type A enterotoxin elicits an enterotoxin-neutralizing antibody response in mice. Infect. Immun. 60:3947-3951. 17.  Chen, C.-Y., Berish, S.A., Morse, S.A., and Mietzner, T.A.  (1993) The iron-binding protein (Fbp) of pathogenic Neisseria functions as a periplasmic transport protein in iron-acquisition from human transferrin.  Molec. Microbiol. 10:311-318. 18.  Miller, M.A., Cloyd, M.W., Liebman, J., Rinaldo, C.R., Islam, K.R., Wang, S.Z.S., Mietzner, T.A., and Montelaro, R.C.  (1993) Alterations in cell membrane permeability by the lentivirus lytic peptide (LLP) of HIV-1 transmembrane protein.  Virology 196:89-100. 19.  Miller, M.A., Mietzner, T.A., Cloyd, M.W., Robey, W.G., and Montelaro, R.C.  (1993) Identification of a calmodulin-binding and inhibitory peptide domain in the HIV-1 transmembrane glycoprotein. AIDS Res. Human Retroviruses 9:1057-1066. 20.  Nowalk, A.J., Tencza, S.B., and Mietzner, T.A. (1994) Coordination of iron by the ferric iron binding protein (Fbp) of pathogenic Neisseria is homologous to the transferrins. Biochemistry 33:12769-12775 21.  Berish, S.A., Chen, C.-Y., Trees, D.L., Subbarao, S., Morse, S.A. and Mietzner, T.A.  (1993) Identification and cloning of a fur regulatory gene in Neisseria gonorrhoeae.  In Pathology and Immunology of Neisseriaceae. Conde-Glez, E.C.J. Morse, S., Rice, P., Sparling, F., Calderon, ed.  Instituto Nacional de Solud Publica.  Cuernavacca, Mexico, pp. 364-368. 22.  Olkowski, J.A., West, M.E., Mietzner, T.A., Chen, C.-Y., Berish, S.A. and Morse, S.A.  (1993) Structure and function of the ferric iron binding protein of Pathogenic Neisseria spp.  In Pathology and Immunology of Neisseriaceae. Conde-Glez, E.C.J. Morse, S., Rice, P., Sparling, F., Calderon, ed.  Instituto Nacional de Solud Publica.  Cuernavacca, Mexico, pp. 410-416. 23.  Tencza, S.B., Miller, M.A., Islam, K., Mietzner, T.A., and Montelaro, R.C. (1995)  Effect of amino acid substitutions on calmodulin binding and cytolytic properties of the LLP-1 peptide segment of HIV-1 transmembrane protein. J. Virol. 69:5199-5202. 24.  Yuan, T., Mietzner, T.A., Montelaro, R.C., and Vogel, H.C.  (1995) Characterization of the calmodulin binding domain of SIV transmembrane glycoprotein by NMR and CD spectroscopy.  Biochemistry 34:10690-10696. 25.  Adhikari, P.A., Kirby, S., Nowalk, A.J., Veraldi, K.L., Schryvers, A.B., and Mietzner, T.A. (1995)  Biochemical characterization of a Haemophilus influenzae periplasmic iron transport operon.  J. Biol. Chem. 270:24719-24726. 26.  Adhikari, P., Berish, S.A., Nowalk, A.J., Veraldi, K.L., Morse, S.A., and Mietzner, T.A. (1996) The fbp ABC transporter of Neisseria gonorrhoeae complements periplasm to cytosol iron transport in Escherichia coli.  J. Bacteriol. 178:2145-2149. 27.  Mietzner, T.A. and Cohen, M.  (1997) Vaccines against N. gonorrhoeae infection.  In:  The molecular approach to new and improved vaccines. Levine, M.M., Kaper, J., et al, eds., Chapter 53, Marcel Dekker, Inc., New York, N.Y. 28.  Mietzner, T.A., Tencza, S.B., Vaughan, K.G., Adhikari, P.A., and Nowalk, A.J. (1997).  Periplasm-to-cytosol free Fe3+ transporters of pathogenic gram-negative bacteria.  In Bacterial Infection:  Close encounters at the host pathogen interface, Vogt, P.K. and Mahan, M.J., eds.  Curr. Top. Microbiol. Immunol. 225:  113-135. 29.  Tencza, S.B., Mietzner, T.A., and Montelaro, R.C. (1997)  Calmodulin-binding function of LLP segments from the HIV type 1 transmembrane protein is conserved among natural sequence variants.   AIDS Res. Hum. Retrovir. 13: 263-269. 30.  Bruns,C.M.  Nowalk, A.J., Arvai, A.S., McTigue, M.A., Vaughan, K.G., Mietzner, T.A., and McRee, D.E. (1997). Structure of Haemophilus influenzae Fe+3-binding protein reveals convergent evolution within a superfamily. Nat. Struct. Biol. 4: 919-924. 31.  Tencza, S.B., Douglas, J.D., Creighton, D.J. Jr., Montelaro, R.C., and Mietzner, T.A. (1997) Novel antimicrobial peptides derived from human immunodeficiency virus type 1 and other lentivirus transmembrane proteins. Antimicrob. Agents  Chemother. 41:2394-2398. 32.  Comardelle, A.M., Norris, C.H., Plymale, D.R., Gatti, P.J., Choi, B., Fermin, C.D., Haislip, A.M., Tencza, S.B.,Mietzner, T.A., Montelaro, R.C., and Garry, R.F.  (1997)  A synthetic peptide corresponding to the carboxy terminus of human immunodeficiency virus type 1 transmembrane glycoprotein induces alterations in the ionic permeability of Xenopus laevis oocytes. AIDS Res. Hum. Retrovir.13:1525-1532. 33.  Nowalk, A.J., Vaughan, K.G., Day, B.,Tencza, S.B. and Mietzner, T.A. (1997)  Metal-dependent conformers of the ferric ion binding protein, Fbp. Biochemistry 36:13054-13059. 34.  Beary,T.P., Tencza, S.B., Mietzner, T.A., and Montelaro, R.C. (1998) Interruption of T-cell signal transduction by lentivirus lytic peptides from HIV-1 transmembrane protein. J. Peptide Res.  51:75-79. 35.  Cakraborty, A., Dyer, K.F., Cascio, M., Mietzner, T.A., and Tweardy, D.J.  (1999)  Identification of a novel STAT3 recruitment motif within the granulocyte colony-stimulating factor receptor. Blood 93:15-24. 36.  Tencza, S.B., Creighton, D.J. Jr., Yaun, T., Vogel, H.J., Montelaro, R.C. and Mietzner, T.A. (1999) Lentivirus-derived antimicrobial peptides:  Increased potency by sequence engineering and dimerization.  J. Antimicrob. Chemotherap. 44:33-41. 37.  Plymale, D.R., Comardelle, A.M., Fermi, C.D., Martin, D.S. Costin, J.M., Norris, C.H., Tencza, S.B., Mietzner, T.A., Montelaro, R.C., and Garry, R.F. (1999) Concentration-dependent differential induction of necrosis or apoptosis by HIV-1 lytic peptide 1. Peptides 20: 1275-83. 38.  Yaun, T., Tencza, S.B., Mietzner, T.A., Montelaro, R.C., and Vogel, H.J.  (2001)  Calmodulin binding properties of peptide analogs and fragments of the calmodulin binding domain of SIV transmembrane glycoprotein 41. Biopolymers 58:  50-62. 39.  Taboy, C.H., Vaughan, K.G., Mietzner, T.A., Aisen, P., and Crumbliss, A.L. (2001) Fe3+ Coordination and Redox Properties of a Bacterial Transferrin. J. Biol. Chem 276: 2719-2724 40.  Bruns, C.M., Anderson, D.S., Vaughan, K.G., Williams, P.A., Nowalk, A.J., McRee, D.E., and Mietzner, T.A.(2001)  Crystallographic and Biochemical Analysis of the Metal-Free Haemophilus influenzae Fe3+-binding protein.   Biochemistry. 40:15631-7. 41.  Phadke, S.M., Lazarevic, V, Bahr, C.C., Islam, K., Beer-Stolz, D., Watkins,S., Tencza, S.B., Vogel, H., Montelaro, R.C., and Mietzner, T.A. (2002) Lentivirus Lytic Peptide 1 (LLP1) Demonstrates Membrane Purturbative Properties Against Both the Outer and Inner Membrane of Serratia marcescens.  Antimicrobial Agents & Chemotherapy. 46(6):2041-5 42.  Dhungana, S., Taboy, C.H., Anderson, D.S., Vaughan, K.G., Aisen, P., Mietzner, T.A., and Crumbliss, A.L.  (2003). The Influence of the Synergistic Anion on Iron Chelation by Ferric Binding Protein, A Bacterial Transferrin.  Proc. Natl. Acad. Sci. 100(7): 3659-64 43.  Phadke, S.M., Islam, K., Deslouches, B., Stolz, D.B., Watkins, S.C., Montelaro, R.C., Pilewski, J.M., Mietzner, T.A. (2003)  Selective toxicity of engineered lentivirus lytic peptides in a cystic fibrosis airway cell model.  Peptides. 24(8):1099-1107 44.  Roulhac PL, Powell KD, Dhungana S, Weaver KD, Mietzner, T.A., Crumbliss AL, Fitzgerald MC. (2004) SUPREX (Stability of Unpurified Proteins from Rates of H/D Exchange) analysis of the thermodynamics of synergistic anion binding by ferric-binding protein (FbpA), a bacterial transferrin.  Biochem. 43:15767-74 45.  Boukhalfa, H., Anderson, D.S., Mietzner, T.A., Crumbliss, A.L. (2003) Kinetics and mechanism of iron release from the bacterial ferric binding protein nFbp: exogenous anion influence and comparison with mammalian transferrin.  J. Biological Inorganic Chemistry. 8(8):881-92 46.  Gabricevi, A.M., Anderson, D.S., Mietzner, T.A., Crumbliss, A.L. (2004) Kinetics and mechanism of iron(III) complexation by ferric binding protein: the role of phosphate. Biochemistry. 43:5811-9. 47.  Anderson, D.S., Adhikari, P., Nowalk, A.J., Chen, C.Y., Mietzner, T.A. (2004) The hFbpABC Transporter fromHaemophilus influenzae Functions as a Binding Protein Dependent ABC-transporter with High Specificity and Affinity for Ferric Iron.  J. Bacteriol.  186: 6220-6229 48.  Dhungana, S, Anderson D.S., Mietzner T.A.,  Crumbliss A.L. (2004)  Phosphate Ester Hydrolysis is Catalyzed by a Bacterial Transferrin: Potential Implications for in vivo Iron Transport Mechanisms.  J. Inorgan. Biochem. 98:1975-77 49.  Deslouches, B., Phadke, S.M., Cascio, M., Islam, K., Montelaro, R.C., Mietzner, T.A. (2005) De novo generation of cationic antimicrobial peptides:  Influence of length and tryptophan substitution on antimicrobial activity.  Antimicrob. Agent. Chemother. 49:316-22 50.  Phadke, S.M., Deslouches, B., Hileman, S.E., Montelaro, R.C., Wiesenfeld, H.C., Mietzner TA. (2005) Antimicrobial Peptides in Mucosal Secretions: The Importance of Local Secretions in Mitigating Infection.  Journal of Nutrition 135:1289-93 51.  Dhungana S, Anderson DS, Mietzner TA, Crumbliss AL. (2005) Kinetics of iron release from ferric binding protein (FbpA): mechanistic implications in bacterial periplasm-to-cytosol Fe3+.  Biochemistry, 44(28):9606-18 52.  Deslouches B, Islam K, Craigo JK, Phadke SM, Montelaro RC, Mietzner TA. (2005) Activity of the de novoengineered antimicrobial peptide WLBU2 against Pseudomonas aeruginosa in human serum and whole blood: implications for systemic applications.  Antimicrob. Agent. Chemother. 49:3208-16 53.  Issacs CE, Rohan L, Weimin X, Jai JH, Mietzner TA, Hillier SH.  (2006) Inactivation of Herpes Simplex Virus clinical isolates using a combination microbicide.  Antimicrob. Agent. Chemother 50:1063-6 54.  Novak K F, Diamond W J, Kirakodu S, Peyyala R, Anderson KW,  Montelaro RC,  and Mietzner TA (2007)  Efficacy of the de novo-derived antimicrobial peptide WLBU2 against oral bacteria. Antimicrob Agents Chemother51:1837-9. 55.  Deslouches B, Gonzalez IA, DeAlmeida D, Islam K, Steele C, Montelaro RC, and Mietzner TA. (2007).  De novo-derived cationic antimicrobial peptide activity in a murine model of Pseudomonas aeruginosa bacteraemia.  J Antimicrob Chemother 60: 669-72. 56.  Heymann JJ, Weaver KD, Mietzner TA, Crumbliss AL.(2007)  Sulfate as a synergistic anion facilitating iron binding by the bacterial transferrin FbpA: the origins and effects of anion promiscuity.  J Am Chem Soc.  129:  9704-12. 57.  Roulhac PL, Weaver KD, Adhikari P, Anderson DS, DeArmond PD, Mietzner TA, Crumbliss AL, Fitzgerald MC  (2008) Ex vivo analysis of synergistic anion binding to FbpA in Gram-negative bacteria. Biochemistry 47:  4298-305. 58.  Weaver KD, Heymann JJ, Mehta A, Roulhac PL, Anderson DS, Nowalk AJ, Adhikari P, Mietzner TA, Fitzgerald MC, Crumbliss AL. (2008).  Ex vivo analysis of synergistic anion binding to FbpA in Gram-negative bacteria.  Biochemistry 47: 4298-305. 59.  Weaver, K.D, Heymann JJ, Mehta A, Roulhac P.L., Anderson D.S., Nowalk, AJ, Adhikari, P, Mietzner TA, Fitzgerald MC, and Crumbliss AL.. (2008). Ga3+ as a mechanistic probe in Fe3+ transport: characterization of Ga3+ interaction with FbpA. J Biol Inorg Chem 13: 887-98. 60.  Parker Siburt CJ, Roulhac PL, Weaver KD, Noto JM, Mietzner TA, Cornelissen CN, Fitzgerald MC, and Crumbliss AL. (2009)    Metallomics  1: 249-256 61.  Chan YR, Liu JS, Pociask DA, Zheng M, Mietzner TA, Berger T, Mak TW, Clifton MC, Strong RK, Ray P, Kolls JK. (2009)  Lipocalin 2 is required for pulmonary host defense against Klebsiella infection. J Immunol. 182:4947-56. 62.  Heymann JJ, Gabričević M, Mietzner TA, Crumbliss AL.  (2009) Kinetics and mechanism of exogenous anion exchange in FeFbpA-NTA: significance of periplasmic anion lability and anion binding activity of ferric binding protein A. J Biol Inorg Chem  1007: s00775-009. 63.  Skinner, MC, Kiselev, AO, Isaacs CE, Mietzner TA, Montelaro RC, Lampe MF. (2010) Evaluation of WLBU2 peptide and 3-O-octyl-sn-glycerol lipid as active ingredients for a topical microbicide formulation targetingChlamydia trachomatis. Antimicrob. Agents Chemother. 54:627-36 64.  Weaver KD, Gabričević M, Anderson DS, Adhikari P, Mietzner TA, and Crumbliss AL. (2010) The role of citrate and phosphate anions in the mechanism of iron(III) sequestration by ferric binding protein: Kinetic studies of holoprotein formation of wild type and binding variants of FbpA.  Biochemistry 49:6021-32 II.  Reviews &Chapters, Edited Books and Patents: A.   Invited Reviews & Chapters 1.    Schoolnik, G.K. and Mietzner, T.A.  (1990) Vaccine development and the molecular pathogenesis of gonorrhea.  In:  The molecular approach to new and improved vaccines.  Woodrow, G.C. and Levine, M.M., eds.  Marcel Dekker, Inc., New York, N.Y.  pp. 565-597. Morse, S.A., Lammel, C.J., Schalla, W.O., Mietzner, T.A. and Brooks, G.F. (1989)  Serum and vaginal antibodies against the major iron-regulated protein in women with pelvic inflammatory disease or uncomplicated infection.  In:  Vaccines for Sexually Transmitted Diseases.  Meheus, A. and Spier, R.E., eds. Butterworths, London, pp. 45-50. 2.    Ho, A.S., Mietzner, T.A., Smith, A.J. and Schoolnik, G.K.  (1990) Isolation and biochemical properties of the pili of Aeromonas hydrophila.  Advances in Research on Cholera and Related Diarrheas, Vol. 10. 3.    Sinha, A.A., Mietzner, T.A., Lock, C.A. and McDevitt, H.O.  (1993) Fluorescence-activated cell sorter analysis of peptide major histocompatibility complex association.  In: Molecular Mechanisms of Immunological Self-Recognition.  Alt, F.W. and Vogel, H.J., eds.  Academic Press, Inc., Orlando. 4.    Mietzner, T.A. and Morse, S.A. (1994) The role of iron-binding proteins in the survival of pathogenic bacteria.  Ann. Rev. Nutr. 14:471-493. 5.    Mietzner, T.A. and Cohen, M.  (1997) Chapter 53.  Vaccine development and the molecular pathogenesis of gonorrhea.  In:  The molecular approach to new and improved vaccines, 2nd edition.  Woodrow, G.C., Levine, M.M., Kaper, J.B., Cobon, G.S., eds., pp. 817-842. Marcel Dekker, Inc., New York, N.Y. 6.    Mietzner, T.A., Tencza, S.B., Vaughan, K.G., Adhikari, P.A., and Nowalk, A.J. (1998).  Perplasm-to-cytosol free Fe(III) transporters of pathogenic gram-negative bacteria Current Topics in Microbiology & Immunology 225:113-35. 7.    Mietzner, T.A., Thomas, C.E., Hobbs, M.M. and Cohen, M.S.  (2004) Vaccines against N. gonorrhoeae infection.  In:  The molecular approach to new and improved vaccines. Levine, M.M., Kaper, J., et al, eds., Chapter 63, pp. 755-773. Marcel Dekker, Inc., New York, N.Y. 8.    Mietzner, T.A. and Morse, S.A.  (2005) NEISSERIAE, In: Borriello, S.P., Murray, P.R and Funke, G. (eds) Topley & Wilson's Microbiology and Microbial Infections, 10th edition, Bacteriology vol 2. London: Hodder Arnold, pp. 1270-1300. 9.     Mietzner, T.A. (2009) Chapter 3:  Classification of Bacteria. In: Jawetz, Melnick & Adelberg’s Medical Microbiology, 25ed (Brooks, Carroll, Butel, Morse, and Mietzner, eds.) McGraw-Hill, New York, NY. 10.  Mietzner, T.A. (2009) Chapter 7:  Microbial Genetics. In: Jawetz, Melnick & Adelberg’s Medical Microbiology, 25ed (Brooks, Carroll, Butel, Morse, and Mietzner, eds.) McGraw-Hill, New York, NY. B.  Edited Books: 1.   McClane, B.A. and Mietzner, T.A., eds.; Dowling, J.A., and Phillips, B.A., associate eds (1999) Microbial Pathogenesis:  a principles oriented approach.  Fence Creek Publishing, Philadelphia, PA.—Still in E-print 2.   Brooks GF, Carroll KC, Butel JS, Morse SA, and Mietzner TA, eds. (2009) Jawetz, Melnick & Adelberg’s Medical Microbiology, 25thed (Brooks, Carroll, Butel, Morse, and Mietzner, eds.) McGraw-Hill, New York, NY. C.  Patents 1.  Mietzner, T.A., Morse, S.A.. 1997.  Major iron-regulated protein of Neisseria gonorrhoeae and its use as vaccine.  Oregon State Board of Higher University. Patent # 4,681,761 2.  McClane, B.A.,  Hanna, P.C., Mietzner, T.A.  Clostridium perfingens type a enterotoxin toxoid and methods of preparation and use as a vaccine and therapeutic agent. Assignee: University of Pittsburgh.   Patent # 5,695,956 3.  Montelaro, R.C., Tencza, S.B., and Mietzner, T.A. 1997.  Antimicrobial peptides.  Assignee:  University of Pittsburgh.  Application # 786748 4.  Montelaro, R.C., Tencza, S.B., and Mietzner, T.A. 1999. Antimicrobial peptides.  Assignee:  University of Pittsburgh.  Application #5945507 5.  Montelaro, R.C., and Mietzner, T.A. 2002. Virus derived antimicrobial peptide.  Assignee:  University of Pittsburgh.  Application #20020169279 6.  Montelaro, R.C., and Mietzner, T.A. 2002. Virus derived antimicrobial peptide.  Assignee:  University of Pittsburgh.  Application #: 20030036627 TEACHING University of Pittsburgh.  A detailed teaching summary of my 19 year career (for which I have received consistently high evaluations) is available upon request.  Most notably I have: a)   Co-directed or participated in the Medical Microbiology course given to freshmen medical students at the University of Pittsburgh. b)   Was instrumental in developing the problem based learning (PBL) component of the current PITT medical school curriculum. c)   Written and edited two microbiology textbooks broadly used by international medical students for studying in the area of microbiology. d)   Developed a two week course in Medical Microbiology that I have taught to freshmen dental students at the Arizona School of Dentistry and Oral Health (2002-present). LECOM at Seton Hill. a)    Instruct first year medical students in the basic sciences, particularly microbiology and immunology, using problem-based learning techniques with approximately 18 hours per week of in-class time b)    Develop board-style exam questions in immunology and microbiology c)    Co-instruct MS-1 histology course d)    Advise first and second year medical students e)    Involvement in a variety of faculty development seminars on lecturing, effective question writing and conflict resolution PROFESSIONAL Awards—Recent a)    Pitt Innovator Award, 2005.  Given by the University of Pittsburgh Provost in recognition of developing my research from the laboratory to a commercially viable product. b)    Outstanding Human Science Instructor, 2009.  Voted by students and faculty at the A.T. Still University, Arizona School of Dentistry and Oral Health. Graduate Students and Post Doctoral Fellows Directly Supervised— 1.    Harold Wiesenfeld, MD, OB/Gyn fellow 2.    Mark Miller, Post Doctoral fellow 3.    Pratima Adhikari, PhD, Post Doctoral fellow 4.    Brendon Wahlberg, PhD student 5.    Andrew Nowalk, MD/PhD student 6.    Sarah B. Tencza, PhD, Post-Doctoral Fellow 7.    Heather Bittner, PhD student 8.    Kristen Veraldi, MD/PhD student 9.    Marjori Bucher, PhD, Post-Doctoral fellow 10.  Kevin G. Vaughan, MD/PhD student 11.  Damon S. Anderson, PhD student 12.  Shruti M. Phadke, MD, Pediatric Pulmonology fellow 13.  Sunil Kapoor, MD, Pediatric Pulmonology fellow 14.  Dalip Palinkovik, MD, Orthopedic fellow 15.  William Keough, MD, Infectious Disease fellow 16.  Anthony Deslouches, MD/PhD Student 17.  Ivan A. Gonzolez, MD, Pediatric Infectious Disease Fellow Thesis Committees Served On— 1.    Patricia Post (Dr. Khan, advisor).  PhD thesis completed 1992 2.    Renee Collie (Dr. McClane, advisor).  Master’s thesis completed 1996 3.    John Kun (Dr. McClane, advisor)  PhD thesis completed 1998 4.    John Perozich (Dr. Hempel, advisor) PhD thesis completed 1999 5.    Andrew Nowalk (Dr. Mietzner, advisor) PhD thesis completed 1997 6.    Heather Bittner (Dr. Mietzner, advisor) Withdrew from Grad School to enter Med School 7.    Michelle Raabe (Dr. Montelaro, advisor) PhD thesis completed 1998 8.    John Leite (Dr. Cascio, advisor) PhD thesis completed 2000 9.    Ana Vallor (Dr. Hillier, advisor) 10.  Michelle Micheletti (Dr. Hillier, advisor) 11.  Anna Nolar (GSPH, Dr. Harrison, advisor) 12.  Larysa Howe (GSPH, Dr. Montelaro, advisor) 13.  Vandana Kalil (Dr. Montelaro, advisor) 14.  Damon S. Anderson (Dr. Mietzner, advisor) 15.  Berthony Deslouches (Drs Montelaro/Mietzner, co-advisor) 16.  Stephanie Goode (Dr. Cascio, advisor) 17.  Derrick Fisher (Dr. McClane, advisor) 18.  James Smedly (Dr. McClane, advisor) 19.  Hans Wildschute (Dr.Hatfull, advisor) 20.  Tara Washko (Dr. Montelaro, advisor)
TOP