1. HPLC analysis of Dopamine, Norepinephrine,
Serotonin and their metabolites in mice with a defective FGFR1 receptor. This project applies to
both Schizophrenia and Parkinson disease.
2. HPLC analysis of human serum samples following
3. HPLC analysis of acetominophen metabolites in a liver toxicity
4. Synthesis and toxicity testing of the dopamine oxidation product, DOPAL. There is
speculation that DOPAL may be a contributor to the neuronal degeneration associated with Parkinson's
disease. The goal of this project is to determine its toxicity to cells in culture and more
specifically to substantia nigra cells in vivo.
5. Real-Time Three-Dimensional analysis
using multiple-oblique illumination to study the contacts between GnRH Immunoreactive elements and
other peptidergic systems.* Previous studies revealed numerous juxtapositions between GnRH
immunoreactive elements and other peptidergic systems in the human diencephalon. These close
associations may be functional synapses and may represent direct influence of various
neurotransmitter systems on the human GnRH release. Due to the long post-mortem time, justification
of these contacts as real synapses utilizing electron microscopy is virtually impossible. However,
close examination of the peptidergic-GnRH juxtapositions with multi oblique illumination can yield
a 3D picture of the contacting elements. Analyzing this 3D image, contacts between the
neurotransmitter systems can be verified, excluding superimpositions of the juxtaposing
* - Denotes projects of the 3-D Microscopy Center within the B.R.L.